Solution behaviour of Chromobacter viscosum and Pseudomonas sp. lipases. No evidence of self-association.

نویسندگان

  • N J Simpkin
  • S E Harding
  • M P Tombs
چکیده

1. The size of two bacterial lipases was studied by SDS/PAGE, sedimentation velocity and sedimentation equilibrium to test for possible self-association behaviour. 2. Mr values of selected lipases were obtained from SDS/PAGE and sedimentation-velocity measurements, together with an absolute determination by sedimentation equilibrium 3. The Mr values obtained in a variety of aqueous solvents indicate that lipases do not self-associate in solution, suggesting the absence of surface hydrophobic patches.

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عنوان ژورنال:
  • The Biochemical journal

دوره 273 ( Pt 3)  شماره 

صفحات  -

تاریخ انتشار 1991